Banca de DEFESA: ARIANE TEIXEIRA DOS SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ARIANE TEIXEIRA DOS SANTOS
DATA : 28/02/2018
HORA: 08:30
LOCAL: Sala de Aula II - PPGCF - Faculdade de Farmácia
TÍTULO:

ESTUDO FITOQUÍMICO E AVALIAÇÃO DA TOXICIDADE E DO EFEITO ANTI-INFLAMATÓRIO DO EXTRATO DA CASCA DA CASTANHA DE CAJÚ (Anacardium occientale) NO MODELO DE ARTRITE AGUDA E CRÔNICA EM RATOS

PALAVRAS-CHAVES:

Anacardiaceae, anacardic acid derivatives; inflammation; industrial waste

PÁGINAS: 177
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Rheumatoid arthritis is an autoimmune disease, its progression is accompanied by pain, edema and bone degeneration. Oil extracted from cashew nuts (Anacardium occidentale) has been the subject of recent researchs motivated by its potential biological effect. Preclinical studies have already demonstrated evidence of several activities, which highlights its antioxidant, anti-inflammatory and anti-tumor effects. Therefore, the goal of this work was to isolate and identify the main markers of the extract, as well as to evaluate the anti-inflammatory effect in the acute and chronic in vivo model and the toxicity of this extract. From the extract of the oil of the chestnut bark of A. occidentale, it was possible to isolate three substances, being characterized as 6- [8 (Z), 11 (Z), 14-pentadecatrienyl] salicylic acid, 6- [8 ), 11 (Z) -pentadecadienyl] salicylic acid and 6- [8 (Z) -pentadecenyl] acid. Pretreatment with the M3 extract (50 and 100 mg / kg, ρ <0.001) revealed a significant reduction of edema in zymosan-induced arthritis, with consequent decrease in leukocyte influx. In addition, M3 extract (50 and 100 mg / kg, ρ <0.001l) caused a significant reduction of myeloperoxidase. In mice, oral administration of the M3 extract (25, 50 and 100 mg / kg, ρ <0.001) showed a significant anti-inflammatory activity in the zymosan-induced air pocket model, evidenced by the reduction in leukocyte influx and oxidative stress markers myeloperoxidase and malonaldehyde. The results also showed a significant decrease in the release of IL-1β. Post-treatment of the extract M3 (25, 50 and 100 mg/kg, ρ <0.001) revealed a significant reduction of paw edema in chronic arthritis induced by CFA. However, only doses of 50 and 100 mg/kg were able to significantly reduce MPO and MDA enzyme levels, as well as cytokines TNF-α and IL-1β ρ <0.001. Acute toxicity of M3 extract (2000 mg/kg, oral) did not produce any behavioral changes in the biochemical and hematological parameters. The biochemical and hematological analyzes of the subchronic toxicity test (50 and 100 mg / kg, orally) did not indicate any change from supplementation with the M3 extract. The results demonstrate that the extract M3 exhibits anti-inflammatory effect in acute and chronic model and this effect may be related to the inhibition of pro-inflammatory cytokines and the migration of neutrophils.

MEMBROS DA BANCA:
Externo ao Programa - 2276354 - LEANDRO DE SANTIS FERREIRA
Externo à Instituição - RITA DE CÁSSIA RAMOS DO EGYPTO QUEIROGA - UFPB
Presidente - 1490222 - SILVANA MARIA ZUCOLOTTO LANGASSNER