Banca de DEFESA: EMANUELL DOS SANTOS SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : EMANUELL DOS SANTOS SILVA
DATA : 08/03/2018
HORA: 14:00
LOCAL: Sala de Aula II - PPGCF - Faculdade de Farmácia
TÍTULO:

NANOPARTÍCULAS POLIMÉRICAS BIODEGRADÁVEIS DE POLI (ÁCIDO LÁCTICO-CO-GLICÓLICO) FUNCIONALIZADAS PARA INCORPORAÇÃO DE PEÇONHA DE Bothrops jararaca

PALAVRAS-CHAVES:

Bothrops jararaca, immunoadjuvants, PLGA nanoparticles, nanobiotechnology

PÁGINAS: 98
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Snake-envenoming represents a worldwide public health issue. Suitable technologies have been investigated for slow release of encapsulated recombinant or native proteins capable of inducing antibody production. The search for new immunoadjuvants and vaccines expands the alternatives and improves the antivenom therapeutics. Nanotechnology is associated to antivenom serum improvement once venom-loaded nanoparticles modulate the protein release and activating immune response to produce specific antibodies. Polymeric nanoparticles are colloidal dispersions with particle size generally lower than 300 nm which have been used as drug delivery systems for bioactive macromolecules. The aim of this study is obtain and characterize biodegradable cationic nanoparticles to proteins loading. The sub 200 nm poly (lactic-co-glycolic acid) (PLGA) nanoparticles were produced and functionalized with hyper-branched polyethylenimine (PEI) using the nanoprecipitation technique. The parameters assessed by dynamic light scattering, zeta potential measurements and microscopy assays were monitored to establish a suitable formulation. Small-sized (100–200 nm) and spherical particles with protein association efficiency of about 100% were reached. The high protein loading efficiency, electrophoresis and zeta potential results demonstrated that Bothrops jararaca venom were adsorbed on particle surface, which remained as a stable colloidal dispersion (over 6 weeks). A slow release protein profile was observed admitting the release kinetics by parabolic diffusion. The in vivo studies demonstrated that venom-loaded nanoparticles were able to produce similar immune response when compared with aluminum hydroxide, an immunoadjuvant widely used in immunization protocol. Thus, the cationic nanoparticles as carrier to bioactive molecules was successfully developed and demonstrated to promising immunoadjuvant and a novel nanocarrier for protein or nucleic acids release.

MEMBROS DA BANCA:
Presidente - 1639820 - ARNOBIO ANTONIO DA SILVA JUNIOR
Externo à Instituição - DENISE VILARINHO TAMBOURGI - IB/SP
Interno - 1178187 - ERYVALDO SOCRATES TABOSA DO EGITO