Banca de DEFESA: MARIA APARECIDA DE ARAUJO PEREIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : MARIA APARECIDA DE ARAUJO PEREIRA
DATA : 15/01/2018
HORA: 09:00
LOCAL: Sala de Aula II - PPGCF - Faculdade de Farmácia
TÍTULO:

NANOPARTÍCULAS LIPÍDICAS SÓLIDAS DE 1,3-DIESTEARIL-2-OLEIL-GLICEROL FUNCIONALIZADAS COM CARGA POSITIVA PARA LIBERAÇÃO MODIFICADA DE PROTEÍNAS

PALAVRAS-CHAVES:

Nanotechnology; Modified Release Systems; Proteins.

PÁGINAS: 121
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

Solid lipid nanoparticles (SLN) are applied colloidal systems as carriers of drugs and biomolecules acting to increase stability and the ability to cross biological barriers. The delivery of proteins as a therapeutic constituent in formulations is a major challenge for the pharmaceutical industry in view of the physico-chemical limitations of these components. In the present study, the triglyceride 1,3-distearyl-2-oleyl-glycerol (TG1) was chosen as the lipid matrix to obtain the particles due to a potential healing effect. The aim of the present study was to develop and characterize SLN from TG1 (SLNTG1) for potential incorporation of proteins and biomolecules. The particles were obtained by the emulsification method with solvent evaporation and characterized by their size, polydispersity and zeta potential using the technique of light scattering (Dynamic Light Scattering). Successful cationic SLNTG1 were obtained with sizes below 300 nm, polydispersion in the 0.2 range and positive zeta potential. Infrared analyzes (FTIR-ATR) proved the interactions between the constituents in the samples. Studies of Atomic Force Microscopy (AFM) found spherical morphology of the particles. High concentrations of BSA protein incorporation were achieved with the cationic NLSTG1, approximately 98%. Detection of BSA by polyacrylamide gel electrophoresis (SDS-PAGE) confirmed the presence of BSA in SLNTG1 and efficacy in incorporation. Cell viability assays have demonstrated the biocompatibility of cationic SLNTG1 and a significant decrease in PEI toxicity in the functionalized nanoparticles and after addition of BSA in SLNTG1. In the in vitro release it was observed that the SLNTG1 were able to prolong the release of the protein longer when compared to the free protein, reaching on maximum release in 6 hours.

MEMBROS DA BANCA:
Presidente - 1639820 - ARNOBIO ANTONIO DA SILVA JUNIOR
Interno - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA
Externo à Instituição - ANGELO RONCALLI ALVES E SILVA - UNIFOR
Externo à Instituição - CYPRIANO GALVAO DA TRINDADE NETO - UnP