Banca de DEFESA: RANIERY DE OLIVEIRA SANTANA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : RANIERY DE OLIVEIRA SANTANA
DATA : 25/08/2017
HORA: 09:00
LOCAL: SALA 2 DO PPgCF
TÍTULO:

Susceptibility of Triatoma brasiliensis (Hemiptera: Reduviidae, Triatominae) to
infection by Trypanosoma cruzi isolates (Kinetoplastida, Trypanosomes) of Rio
Grande do Norte

PALAVRAS-CHAVES:

Susceptibility, Mixed infection, T. brasiliensis, Biodems, kDNA

PÁGINAS: 70
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

The susceptibility of Triatoma brasiliensis to infection by Trypanosoma cruzi DTUs I, II and III found in the State of Rio Grande do Norte was evaluated experimentally. Nymphs were fed using direct and indirect xenodiagnosis with the isolates 3188 (TcI), RN79/RN2 (TcII) and Pl1.10.14 (TcIII) in simple or mixed infections. The intestinal contents were evaluated by direct exam, xenoculture and kDNA polymerase chain reaction (PCR). Then, the characterization of isolates was used the cytochrome oxidade subunit 2 gene (COII), 24Sα ribosomal DNA gene (rDNA) and the spliced-leader intergenic region of the mini-exon gene (SL-IR) as markers. In the direct xenodiagnosis, the direct exam presented 30.0%(15/50) of positivity, the xenoculture 18.0%(9/50) and the kDNA PCR 88.0% (44/50). Meanwhile, the indirect xenodiagnosis showed 5.4%(3/56) of positivity to the direct exam, 25.0%(14/56) to xenodiagnosis and 41.1%(23/56) to kDNA PCR. Comparing the techniques, the direct xenodiagnosis showed better results than the indirect procedure. The PCR identified the T. cruzi in both types of infection, with higher positivity than direct exam and xenoculture. With respect the molecular characterization, the re-isolates showed the same profile before and after the simple infections. In the mixed infections were verified two or only one isolate. The data demonstrated the susceptibility of T. brasiliensis to DTUs I, II and III, with high rates in simple and mixed infections. The vector can resist mixed infections up to three isolates, reinforcing the importance of T. brasiliensis to the epidemiology of T. cruzi infection in Rio Grande do Norte.

MEMBROS DA BANCA:
Presidente - 1218940 - ANTONIA CLAUDIA JACOME DA CAMARA
Externo à Instituição - HELENA KEIKO TOMA - UFRJ
Externo ao Programa - 1715271 - RENATA ANTONACI GAMA