Banca de DEFESA: IAPONIRA ROQUE BARBOZA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : IAPONIRA ROQUE BARBOZA
DATA : 29/11/2016
HORA: 14:00
LOCAL: SALA DE AULA I PPGCF
TÍTULO:

NANOPARTÍCULAS  DE  ÁCIDO  POLI LÁTICO-CO-GLICÓLICO  FLUORESCENTES  PARA  POTENCIAL  VETORIZAÇÃO  DE  FÁRMACOS  E  BIOMOLÉCULAS  ATRAVÉS  DE  BARREIRAS  BIOLÓGICAS 

PALAVRAS-CHAVES:

nanoparticles, functionalization, Doxorrubicin, nanoprecipitation, fluorescent.

PÁGINAS: 115
GRANDE ÁREA: Ciências da Saúde
ÁREA: Farmácia
RESUMO:

The mainly limitation of cancer therapy is the nonspecific biodistribution of the anticancer drugs for the affected tissues. This way is not different for the doxorubicin (DOX), a highly potent antineoplastic used against a wide spectrum of solid tumors. Cationic functionalized polymeric nanoparticles (NPs) have demonstrated superior ability to overcome biological barriers, enhancing the efficacy and reducing side effects. The purpose of this study was to prepare positively charged poly (lactide-coglycolide) nanoparticles linked to a fluorescent dye, for the potential treatment and efficacy monitoring of anticancer drugs. The doxorubicin hydrochloride was used as template molecule, hyperbranched polyethileneimine (PEI) 25 kDa was used as cationic agent. . The nanoprecipitation method was optimized to obtain small and narrow-sized biodegradable and biocompatible DOX-loaded NPs. In this way, different surfactants were tested, such as surfactant polysorbate 80 and 85, sorbitan monooleate 80, polyvinyl alcohol (PVA), poloxamer 188 and 407. Different PLGA/surfactant, as well as PLGA/PEI ratio were also tested. To obtain fluorescent labeled NPs, the copolymer was covalently bounded to fluorescein isothiocyanate (FITC) NP preparation. The particles stabilized with poloxamers 188, using PEI to PLGA weight ratio 1:5 exhibited uniform size distribution around 60 nm and zeta potential of +17.1 mV, and biocompatible for the vero cell. The fluorescence was also proved by fluorescence microscopy images, Fourier transforms Infrared spectroscopy, and flow cytometry. The experimental data discussed in this approach demonstrated the feasibility of doubly functionalized biocompatible PLGA nanoparticles for the potential cancer therapy and for monitoring drug efficacy by preliminary studies in tumor liver cell lines (HepG2) and kidney (786-0) and non-tumor kidney cells (HEK293T).

MEMBROS DA BANCA:
Presidente - 1639820 - ARNOBIO ANTONIO DA SILVA JUNIOR
Externo ao Programa - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externo à Instituição - ANA MARIA DA SILVA MAIA - UFT