Banca de DEFESA: MARIA LUCIA DA SILVA CORDEIRO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : MARIA LUCIA DA SILVA CORDEIRO
DATE: 24/10/2023
TIME: 13:30
LOCAL: https://meet.google.com/dxs-krqu-ejb
TITLE:

EXTRACTS OF Talisia esculenta PRESENT COMPOUNDS BIOACTIVES WITH ANTIOXIDANT ACTIVITY AND POTENTIAL ANTI-INFLAMMATORY IN IN VITRO AND IN VIVO MODELS

KEY WORDS:

Sapindaceae; oxidative stress; Tenebrio molitor; medicinal plant; Zebrafish

PAGES: 115
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Química de Macromoléculas
SUMMARY:

Commonly referred to as Pitombeira, Talisia esculenta (Sapindaceae) is an indigenous species endemic to Northeastern Brazil with significant economic and medicinal promise. While the fruit of this plant serves as a food source, its rind remains largely underutilized in pharmacological applications and is often discarded without further biotechnological exploration. Traditional folk medicine employs leaf infusions from T. esculenta as remedies for hip pain, rheumatism, and hypertension. However, comprehensive studies evaluating its pharmacological efficacy, toxicological profile, and phytochemical constituents are notably limited. In this context, the aim of this study was to obtain hydroethanolic extracts and infusions of the leaves and fruit rinds of T. esculenta. Subsequently, it aims to perform its phytochemical characterization, measure its antioxidant and anti-inflammatory potential in vitro and its toxicity and antioxidant capacity in vivo. For this purpose, two animal models will be used, Tenebrio molitor and Zebrafish. The hydroethanolic extract (70%) and the aqueous extract (infusion) were prepared from the fresh leaves and fruit peels of the species, obtaining the hydroethanolic extract of the leaves (HF), infusion of the leaves (IF), hydroethanolic extract of the fruit peels (HC), and infusion of the fruit peels (IC). Firstly, the antioxidant capacity was assessed using various assays: Total Antioxidant Capacity (CAT), DPPH radical scavenging, reducing power, and copper chelation. Cytotoxicity was evaluated through the application of MTT and wound healing assays, while the antioxidant potential was quantified in NIH/3T3 cells. As the extracts IF and HC were selected for further investigation on their effects on T. molitor and phytochemical characterization by quantifying the content of phenolics and flavonoids and analyzing them by CLAE-DAD (High-Performance Liquid Chromatography with Diode-Array Detection). This work also analysed the antioxidant and anti-inflammatory potential of all the T. esculenta extracts on the RAW 264.7 (macrophage strain). In addition, the protective effects of T. esculenta extracts were investigated using models of oxidative stress induced by H2O2 and CuSO4 and ascorbate in zebrafish, based on the analysis of levels of reactive oxygen species (ROS). The assays indicated that both extract types exhibited effective radical scavenging capabilities and a high capacity for copper ion chelation. It was observed that T. esculenta extracts had no effects on MTT reduction or cell migration. Furthermore, these extracts protected cells from oxidative stress induced by both CuSO4 and ascorbate. Survival analysis revealed that IF and HC exhibited no significant toxicity at the evaluated concentrations. Moreover, these extracts mitigated the effects of CuSO4 exposure on T. molitor larvae. Specifically, an increase in larval survival was observed when treated with these extracts compared to the positive control. Additionally, a reduction in melanization was verified in the animals. The CLAE-DAD analyse revealed the presence of compounds mostly from the flavonoid class, and the presence of gallic acid, quercitrin and rutin was identified In RAW 264.7 cell assays, the extracts demonstrated no cytotoxicity as per MTT tests and effectively mitigated oxidative damage induced by H2O2 in macrophages. However, only HC was able to reduce nitric oxide (NO) production in LPS-stimulated macrophages. Moreover, the protective effects of T. esculenta extracts on H2O2 and CuSO4 and ascorbate-induced oxidative stress model in zebrafish were also investigated based on the analysis of ROS levels by fluorescence microscopy. All extracts reduced ROS levels in larvae exposed to H2O2, however a significant reduction was verified for HF, IF and HC, which were statistically equal to the negative control. All samples reduced ROS levels in the CuSO4 and ascorbate-induced stress model. Collectively, these findings suggest that T. esculenta is a promising source of bioactive compounds with potent antioxidant and anti-inflammatory properties, warranting further research for potential biotechnological applications.

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COMMITTEE MEMBERS:
Interna - 1549705 - ADRIANA FERREIRA UCHOA
Externa à Instituição - DAYANNE LOPES GOMES - IFPI
Externa à Instituição - DEBORAH YARA ALVES CURSINO DOS SANTOS - USP
Presidente - 1453487 - KATIA CASTANHO SCORTECCI
Interno - 2962496 - RAFAEL BARROS GOMES DA CAMARA