Banca de DEFESA: CÁSSIO RICARDO DE MEDEIROS SOUZA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : CÁSSIO RICARDO DE MEDEIROS SOUZA
DATE: 09/10/2023
TIME: 10:00
LOCAL: Videoconferência - Link para acesso: meet.google.com/toh-svdw-mui
TITLE:

ANTI-FLAVIVIRIDAE ANTIBODIES IN GUILLAIN-BARRÉ AND CONGENITAL ZIKA SYNDROMES

KEY WORDS:

ZIKV; serological diagnosis; anti-ZIKV antibodies; Guillain-Barré Syndrome; Congenital Zika Syndrome.

PAGES: 103
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

Between 2015 and 2016, Brazil has experienced the major ZIKV epidemics registered until now, which became a public health problem not only because the great number of cases, but mainly by the onset of critical infection-associated clinical conditions, like Guillain-Barré (GBS) and Congenital Zika Syndromes (CZS). The evaluation of serological diagnosis efficacy and antibody response profile are two great issues in ZIKV infection context. Serological diagnosis is mainly performed by detection of anti-NS1 antibodies, but there is evidence about cross-reactivity with antibodies against other flaviviruses, which impairs the ZIKV infection specific diagnosis. Also, urge the importance of study anti-ZIKV antibodies response and kinetics post-infection, to determine the existence of long-term antibodies and its protective capacity. So, the present work focuses in these two issues. First, efficacy NS1-based serology to detection of IgM, total IgG and IgG3 anti-ZIKV and anti-DENV and the application in differential diagnosis of ZIKV infection in SGB was evaluated. The presence of anti-ZIKV and anti-DENV antibodies in patients who developed GBS between 2009 and 2018, residents of DENV endemic area, was registered. The presence of these antibodies in individuals who developed the syndrome before ZIKV introduction indicates the occurrence of cross-reactivity between anti-flaviviridae antibodies. Data indicates IgM and total IgG serology, besides considerable sensitivity, do not present specificity enough to supports differential diagnosis of ZIKV infection. However, anti-NS1 IgG3 serology shows better performance, making possible the identification of ZIKV-associated GBS cases with more efficacy. Next, the presence of anti-NS1, anti-E and neutralizing antibodies against ZIKV and/or DENV, was determined in woman who gave birth children with CZS, evaluated in two distinct periods, 8-24 months post-partum and 25-44 months post-partum, to identify the occurrence of long-term protective antibody response. The results indicate significant presence of anti-NS1, anti-E and neutralizing antibodies in woman evaluated between 8- and 24-months post-partum, as an important sign of previous ZIKV exposition. IN the second group, it was not possible to identify significant presence of anti-NS1 and anti-E antibodies, suggesting time-associated decay of the response. However, the presence of neutralizing antibodies was registered. Anti-ZIKV neutralizing response are related to presence of anti-ZIKV antibodies in first group and, int the second one, this phenomenon was associated with the occurrence of anti-DENV antibodies, suggesting cross-protective activity between anti-ZIKV and anti-DENV antibodies. It is necessary more studies to a better characterization of IgG3 role as specific markers of ZIKV infection, and long-term anti-NS1 and anti-E as anti-ZIKV neutralizing antibodies, to improve serological diagnosis strategies and enforces the knowledge about kinetics and protective role of anti-ZIKV antibodies.

COMMITTEE MEMBERS:
Externo ao Programa - 3010614 - ELIARDO GUIMARAES DA COSTA - nullExterno ao Programa - 1178187 - ERYVALDO SOCRATES TABOSA DO EGITO - nullExterno à Instituição - LUCAS PEDREIRA DE CARVALHO - UFBA
Externo à Instituição - LUIZ BEZERRA DE CARVALHO JR - UFPE
Presidente - 350647 - SELMA MARIA BEZERRA JERONIMO