Banca de QUALIFICAÇÃO: ELISÂNGELA DA COSTA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ELISÂNGELA DA COSTA SILVA
DATE: 22/09/2023
TIME: 14:00
LOCAL: Videoconferencia
TITLE:

TOXICITY ANALYSISOF THE BIOMATERIALHYDROXYAPATITE FUNCTIONALIZEDWITHSTRONTIUMION(Sr-HA)INZEBRAFISH

(Daniorerio).

KEY WORDS:

Hydroxyapatite,Strontium,nanostructuredbiomaterials,embryo biosafety.

PAGES: 65
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

Hydroxyapatite(HA) is an essential inorganic component found in human bone tissue, and its compatibility with biological systems makes it widely applicable in various biomedical fields. The ability to incorporate different ions into the HA structure allows the modification ofitsproperties,makingthemmoresimilartothoseofbonetissueitself. One of these ions that can be incorporated is strontium, known for its ability to stimulate osteogenic activity. However, the biological effects of strontium-functionalized HA nanoparticles (SrHA) are not yet completely understood. To address this gap, an embryotoxicity study using zebrafish (Danio rerio) was performed, following OECD 236 guidelines. Zebrafish embryos were exposed to microspheres containing synthesized nSrHA nanoparticles at temperatures of 5°C and 90°C. for 120 hours. During the test, the mortality rate, developmentalparametersandproductionofreactiveoxygenespécies (ROS) were evaluated. At 168 hpf, behavioral parameters were assessed through motor responses and anxiety-like behavior to verify whether the biomaterials caused neurotoxic effects. The results revealed that the survival rate slightly decreased in the group treated with 5 °C SrHA, and there was a slight increase in ROS production in this group. However, none of the biomaterials caused morphological changes that would suggest toxicity during larval development. Furthermore,behavioraltestsshowednosignificantdifferencesinany of the experimental groups, suggesting that exposure to the tested biomaterials did not have neurotoxic effects. These findings provide valuable information on the safety of HA-based nanostructured biomaterials and highlight their promising applicability in bone tissue repair. With the increasing use of hydroxyapatite-based biomaterials, investigations into the safety of these materials are essential.

COMMITTEE MEMBERS:
Presidente - 1997012 - RAQUEL CORDEIRO THEODORO
Interna - 1674709 - VIVIANE SOUZA DO AMARAL
Externo à Instituição - THIAGO LOPES ROCHA - UFG