Banca de DEFESA: LEIDIANE BARBOZA DA SILVA
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.STUDENT : LEIDIANE BARBOZA DA SILVA
DATE: 22/12/2022
TIME: 09:00
LOCAL: Videoconferência - Link para acesso: https://meet.google.com/frz-onka-svq
TITLE:
EFICÁCIA E SEGURANÇA DO EXTRATO DE SEMENTES DE ANGICO (Anadenanthera colubrina) CONTRA Aedes aegypti E Aedes albopictus, EM CONDIÇÕES DE LABORATÓRIO E DE CAMPO
KEY WORDS:
Botanical extracts; Angico; Arboviruses; Aedes; Vector insect control.
PAGES: 118
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Química de Macromoléculas
SPECIALTY: Proteínas
SUMMARY:
Aedes aegypti (L., 1762) and Aedes albopictus (S., 1894) are vectors of the etiological agents of numerous arboviruses of medical importance. Limitations in the use of chemical insecticides have stimulated the search for effective and ecologically friendly options, such as botanical insecticides. The present study describes the aedicidal potential of Anadenanthera colubrina (Vell.) seed extract at different stages of the vectors' life cycle, its attractive/repellent action on oviposition, partial biochemical characterization and evaluation of cyto- and ecotoxicity. The hot aqueous extract of A. colubrina seeds (EAQA) was obtained by extraction in distilled water (1:10, m/v) for 7 h at 80 °C. The larvicidal effects of EAQA were evaluated in serial dilutions and the lethal LC50 and LC90 concentrations (mg/mL) determined after 24 h and 48 h of exposure. The larvicidal effect was observed for A. aegypti and A. albopictus, recording LC50 and LC90 at 24 h (0.16 and 0.94 mg/mL; 0.36 and 1.28 mg/mL) and 48 h (0. 02 and 0.26 mg/mL; 0.06 and 0.62 mg/mL), respectively. EAQA was pupicid for both insects at concentrations higher than larvicidal concentrations (24 h LC50 ≥ 0.85 mg/mL and 48 h LC50 ≥ 0.53 mg/mL). 0.26 mg/mL was defined as the diagnostic concentration for ovicidal activity and oviposition, showing ovicidal activity for both insects, compromising larval hatching by 76% and reducing adult emergence of surviving larvae by 25%. Under field conditions, EAQA exerts an attractive effect on oviposition. EAQA showed a higher concentration of total carbohydrates (15.5 mg/mL) compared to total soluble protein (2.0 mg/mL) and total phenolic compounds (0.1 mg/mL). EAQA presents bromelain, papain, trypsin and chymotrypsin inhibitors, with enzyme inhibition activity in 90.94%, 68.93%, 68.75% and 9.32%, respectively. No cytotoxicity was observed for tested cell lines (3T3) and (HepG2), nor ecotoxicity for Ceriodaphnia dubia (LC50 = 0.72 mg/mL for 24 h and 0.34 mg/mL for 48 h) and Mysidopsis juniae (LC50 = 0 .87 mg/mL for 24 h and 0.37 mg/mL for 48 h). EAQA has the potential to be an adjuvant in Aedes integrated management strategies due to the versatility of action against different stages of the vectors' life cycle, as well as their cyto- and eco-compatibility.
COMMITTEE MEMBERS:
Presidente - 1549705 - ADRIANA FERREIRA UCHOA
Interno - 2213126 - VALTER FERREIRA DE ANDRADE NETO
Externo à Instituição - JOSÉ ROBERTO DA SILVA - UFRJ
Externa à Instituição - PATRICIA BATISTA BARRA MEDEIROS BARBOSA - UERN