Banca de QUALIFICAÇÃO: LUCIANA FENTANES MOURA DE MELO

ANTIOXIDANT AND IMMUNOMODULATING POTENTIAL OF EXTRACTS AND FRACTIONS OF LEAVES OF Coccoloba alnifolia

Cauaçu; Leaves, ROS, NO reduction, phagocytosis, wound healing, qRT-PCR

Coccoloba alnifolia (Polygonaceae) is a tree from Atlantic Forest. Previously, it was verified the in vitro antioxidant activity from EE and AE Here, it was evaluated the immunomodulatory activity using RAW 264.7 cell line (a macrophage cell). Considering the complexity of inflammation process, it was analyzed the EE and AE effect on ROS production after LPS treatment. . It was verified that both extracts in all concentrations evaluated (100, 250 and 500 µg/mL) were able to reduce ROS production when compared to positive control (with only LPS). Furthermore, the result obtained for EE was similar to negative control (no LPS). The other activity analyzed was the Nitric Oxide (NO) level. It was observed that EE and AE extracts were able to promote the reduction of NO production in all three extract concentrations tested. In addition, it was evaluated the phagocytic activity using the only the 500 μg/mL concentration, and it was verified a reduction on 30% for EE and on 50% for AE. It was also measured the cytokines by flow cytometric and by qRT-PCR. And the results obtained proposed that these extracts may had an immunomodulatory effect. Moreover, it was also analyzed the healing process by the scratch assay using NHI/3T3 cell line (a fibroblast). This assay showed that in 24 h both EE and EA extracts had a 60% wound recovery. Therefore, the data presented here demonstrated that the C. alnifolia EE and AE extracts have a potential immunomodulatory, as well as in vitro wound healing, characteristics important to control the dynamic of inflammation process.