Banca de QUALIFICAÇÃO: LAYSA OHANA ALVES DE OLIVEIRA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : LAYSA OHANA ALVES DE OLIVEIRA
DATE: 04/03/2022
TIME: 14:00
LOCAL: Videoconferencia
TITLE:

In silico analysis of the transcriptome of GG-NER and TC-NER deficient cells in response to oxidative stress

KEY WORDS:

Oxidative stress; Nucleotide excision repair (NER); GG-NER; TC-NER; CSB; XPC.

PAGES: 96
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

The nucleotide excision repair (NER) pathway removes lesions that distort the DNA double helix, such as UV radiation products (pyrimidine dimers and 6,4- photoproducts), benzo[a]pyrene adducts, and chemotherapeutic damage. Although the base excision pathway (BER) is responsible for repairing oxidized DNA damage, studies have demonstrated a role of the NER pathway in the repair of this type of damage. Mutations in genes of the NER pathway give rise to several syndromes, such as xeroderma pigmentosum (XP) complementation group C, caused by mutations in the XPC gene, and Cockayne syndrome (CS), caused by mutations in the CSB gene. Such disorders have the most varied phenotypes and are the focus of this work. NER factors with functions in processes other than repair have already been reported, and the study of these functions can help elucidate the different phenotypes found in XP and CS, for example. Thus, the present work analyzed the transcriptome of cells deficient in CSB (CS1AN) and XPC (XP4PA), cell lines deficient in TC-NER and GG-NER, respectively, treated with hydrogen peroxide (H2O2). The analysis was performed using computational tools in order to investigate the occurrence of different cellular responses to oxidative stress. The results suggest that CSB and XPC are interacting with proteins from other pathways and have a role in transcriptional regulation, as possible interactions of CSB with JUN, HDAC1, MAPK1 were predicted. It is still possible to assume that CSB also participates in VEGF induction and regulates BRCA1 and MDM2. XPC may be involved in the regulation of PIK3CD, TAF1, ELK1 and some miRNAs, such as miR-27a/221/222, as the transcriptome of XP4PA cells showed a large number of up-regulated miRNAs in response to oxidative stress, suggesting a role for XPC in the regulation of these transcripts.

BANKING MEMBERS:
Interna - 1453487 - KATIA CASTANHO SCORTECCI
Presidente - 1507794 - RODRIGO JULIANI SIQUEIRA DALMOLIN
Interna - 1199127 - SILVIA REGINA BATISTUZZO DE MEDEIROS