Banca de DEFESA: JÉSSIKA ALINE DO NASCIMENTO MEDEIROS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JÉSSIKA ALINE DO NASCIMENTO MEDEIROS
DATE: 24/10/2022
TIME: 09:00
LOCAL: Sala de reunião da GEP, na Maternidade Escola Januário Cicco-UFRN / Videoconferência
TITLE:

Characterization of Th17, Th1 and Th2 axis cytokine profile and gene polymorphism in patients with cervical cancer.

KEY WORDS:

Cervical cancer, polymorphism, Th1, Th2 and Th17 profile cytokines

PAGES: 50
BIG AREA: Ciências da Saúde
AREA: Medicina
SUBÁREA: Saúde Materno-Infantil
SUMMARY:

Cervical cancer (CCU) is a public health problem that affects many women around the world and its etiology and pathogenesis are intrinsically linked to Human Papillomavirus (HPV), representing the fourth cause of death among women. Estimates report that about 80% of sexually active women will acquire an infection in their lifetime. It is known that the host's immune system, in most cases, is able to eliminate the HPV virus, but in some cases the patient will develop a persistent infection, which will culminate in the emergence of cervical cancer. Considering the importance of the immune system in this process, it is necessary to understand the cells and mechanisms involved. Therefore, evaluating the T helper cell pattern 1, 2 and 17 (Th1, Th2 and Th17) via cytokine quantification and associating the gene polymorphism of the IL-17-RA17 axis is the objective of the present study. This is a case-control observational study, with retrospective data and samples acquisition, having been collected from 2015 to 2019, and the study population consisted of 428 samples from a group of patients with indication of intraepithelial lesion and cervical cancer at the Cervical Pathology and Oncology outpatient clinic of the Januário Cicco Maternity School and the Hospital Luiz Antônio da Liga Contra o Câncer, they were stratified into 104 (24 %) with cervical cancer (CC), 125 (29 % ) with high-grade lesions, 86 (20%) with low-grade lesions, 104 (24%) were NILM, and 9 (3%) had indeterminate lesions, and the patients identified as NILM were classified as controls in this study. The execution of this work involves carrying out the gene polymorphism of IL17A and IL17RA in the following steps: DNA extraction and quantification; Polymerase Chain Reaction (PCR); and electrophoresis, while cytokine quantification was performed using the BD™ Cytometric Bead Array (CBA) Human Th1/Th2/Th17 cytokine kit. In our series, the data showed that there seems to be no association between the gene polymorphism of the IL-17-RA17 axis and the diagnosis of CC, in addition, in our study, the cytokines presented values below the detection limit, which can be justified by the action of HPV being a little cytopathic virus, imperceptible to the immune system.

COMMITTEE MEMBERS:
Presidente - 3313589 - JANAINA CRISTIANA DE OLIVEIRA CRISPIM FREITAS
Interna - 1801992 - PAULA RENATA LIMA MACHADO
Externa à Instituição - MARCIA EDILAINE LOPES CONSOLARO - UEM