Banca de DEFESA: IASMIM SANTOS MANGABEIRA E SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : IASMIM SANTOS MANGABEIRA E SILVA
DATE: 20/12/2022
TIME: 14:00
LOCAL: Online (Link do Google Meet: https://meet.google.com/gur- wnvf-zzi)
TITLE:

Characterization of SNPs and Microsatellite molecular markers in Penaeus vannamei and proposal of panels for population and association studies.

KEY WORDS:

Aquaculture; shrimp farming; viral diseases; microsatellites; SNPs.

PAGES: 95
BIG AREA: Ciências Biológicas
AREA: Genética
SUMMARY:

Shrimp farming worldwide, and especially in Brazil, suffers profound impacts due to the lack of standardization in production processes and the occurrence of infectious diseases. In this sense, the study of the genome of the shrimp Penaeus vannamei and the development of new tools that allow the selection of animals with the vigor necessary for production is crucial for the success of the activity. Among these tools, the use of molecular markers and their association a trait of interest has shown to be a promising strategy, especially considering that vaccines and treatments are not available for shrimp farming. However, information about molecular markers for P. vannamei is still quite fragmented and many markers are not properly characterized. Thus, this work aimed to identify and characterize in detail single nucleotide polymorphism (SNP) and microsatellite (STR) markers already described in the literature for P. vannamei, and based on this characterization, propose panels of markers with potential application in population and association studies. In Chapter 1, 512 SNPs associated with genes involved in disease resistance were identified and classified in detail. It was observed that most SNPs were present in Toll-like receptor proteins 1 and 3, large and small subunits of hemocyanin and anti-lipopolysaccharide factors 1 and 2, allowing to propose their use as targets in studies of association and genetic improvement of populations of P . In chapter 2, 306 polymorphic STRs were characterized in detail, in order to identify their location in the genome. Among these, 38.8% were considered perfect, with the highest prevalence of dinucleotides (45.3%). The number of uninterrupted repetitions ranged from 3 to 62, with the majority consisting of short repetitions. Putative sequences of 135 STRs were obtained and, after functional alignment, 62 of them were associated with coding genes (mRNA). The identified proteins are mainly related to binding activity (53.5%) and catalytic activity (32.14%). Furthermore, 7 perfect STRs located in DNA coding regions were validated in vitro in a shrimp population. The average number of alleles per locus was approximately five, indicating that there may be different protein isoforms in the population. In conclusion, this work summarizes all the markers already described and characterized for P. vannamei and details panels of SNPs and microsatellites in coding regions. These markers have potential for use in population studies and, above all, for studies of direct phenotype-genotype association, increasing the study of genetic plasticity and genotype phenotype correlation in P. vannamei.

COMMITTEE MEMBERS:
Presidente - 1880243 - DANIEL CARLOS FERREIRA LANZA
Externa ao Programa - 1731226 - KARINA RIBEIRO - UFRNExterna ao Programa - 1997012 - RAQUEL CORDEIRO THEODORO - UFRNExterna à Instituição - ALINE SILVA MELLO CESAR - USP
Externa à Instituição - JULIANA GABRIELA SILVA DE LIMA