Banca de QUALIFICAÇÃO: GIOVANNA MELO MARTINS SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : GIOVANNA MELO MARTINS SILVA
DATE: 17/11/2022
TIME: 08:00
LOCAL: Google Meet
TITLE:

A DEFINIR

KEY WORDS:

A DEFINIR

PAGES: 42
BIG AREA: Ciências Biológicas
AREA: Genética
SUMMARY:

Coronaviruses (CoVs), from the Coronaviridae family, can infect a variety of hosts, producing symptoms and illnesses that range from the common cold to more serious or fatal conditions. Before the emergence of SARS CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), which causes COVID-19 (Coronavirus Disease 2019), only a few CoVs were known to cause disease in humans, from which SARS -CoV and MERS-CoV were the only viruses in the family associated with high mortality rate. It is possible that the emergence of new viruses from this family may occur due to the co-infection of multiple CoVs in the same natural host, favoring genetic recombination, and the high rates of mutations that occur due to the instability of RNA-dependent RNA polymerases. Considering the importance of better investigating the behavior of these viruses, the present project aims to identify viral sequences from Coronaviridae family in hosts’ metagenome using bioinformatics tools (fastp, Kaiju, BWA and SPAdes). It is expected that with this analysis will be possible to identify relevant biological, taxonomic and genetic information (sequence diversity), which can better describe virus-host relationship. There are 46 CoVs distributed in five genera and associated with hundreds of hosts. From these, only 68 have reference genomes and only these species were selected to search for sequences in the public database from SRA (Sequence Read Archive/NCBI), using keywords. In this way, we found 24367 sequencing products belonging to several projects. In the first part of the work, contemplating the quality control analysis (fastp 0.20.1) and taxonomic classification (Kaiju 1.7.4), 836 samples were found positive for CoVs, which were all negative when submitted to retest by another tool (BWA). These results suggest that the investigated samples did not have coronavirus, only conserved and similar sequences between organisms, which was overestimated by the Kaiju tool.

COMMITTEE MEMBERS:
Externa à Instituição - STELA MIRLA DA SILVA FELIPE ACÁCIO - UECE
Externo à Instituição - GLORIA REGINA FRANCO - UFMG
Externo ao Programa - 1056188 - THIAGO BRUCE RODRIGUES - null