Banca de QUALIFICAÇÃO: IASMIM SANTOS MANGABEIRA E SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : IASMIM SANTOS MANGABEIRA E SILVA
DATE: 17/12/2021
TIME: 13:00
LOCAL: Online (remota)
TITLE:

A DEFINIR

KEY WORDS:

Aquaculture; shrimp farming; viral diseases; microsatellites; SNPs.

PAGES: 91
BIG AREA: Ciências Biológicas
AREA: Genética
SUMMARY:

Brazilian shrimp farming has suffered profound impacts as a result of different infectious diseases in recent decades; especially in the states of Rio Grande do Norte and Ceará. In this sense, the study of the genome of the shrimp Penaeus vannamei is of fundamental importance to understand essential mechanisms for their survival and, thus, to develop effective tools to control viral outbreaks. Among these tools, the use of molecular markers associated with characteristics of interest in production systems has already shown to be a promising strategy, however, the information on these markers is still quite limited. Thus, the study aimed to identify and characterize in detail the single nucleotide polymorphism (SNP) and microsatellite (STR) markers already available and validated in the literature for P. vannamei, in addition to developing a panel of microsatellite markers that could be applied to assess genetic variability in marine shrimp breeding programs. In Chapter 1, 512 SNPs related to disease resistance were identified and classified in detail. It was observed that most SNPs were present in Toll-like receptor proteins 1 and 3, large and small subunits of hemocyanin and anti-lipopolysaccharide factors 1 and 2, allowing to propose their use as targets in association studies involving resistance in P. vannamei. In chapter 2, it was identified that 306 polymorphic STRs were validated in population studies. Of these, 119 STRs were perfect, with the highest prevalence of dinucleotides (45.3%). The number of uninterrupted repetitions ranged from 3 to 62, with the majority consisting of short repetitions. In the functional annotation, 62 sequences (inserted STRs) in which they had similarity could be attributed to predicted genes. The identified proteins are mainly related to binding (58.3%) and catalytic activity (31.25%). In chapter 3, 28 perfect STRs were selected for a population study. After amplification of these markers for validation in the population, 15 STRs showed a satisfactory band pattern, with 55o C being the best annealing temperature. After standardizing the PCR reactions, 6 STRs that showed greater polymorphism and better visualization of amplification were chosen for testing in 50 animals. Given the previous results obtained, it can be concluded that the polymorphic microsatellite loci described here are potential markers for population studies in Penaeus vannamei.

BANKING MEMBERS:
Externa ao Programa - 1731226 - KARINA RIBEIRO
Presidente - 1997012 - RAQUEL CORDEIRO THEODORO
Interna - 1674709 - VIVIANE SOUZA DO AMARAL