Banca de QUALIFICAÇÃO: GILDACIO PEREIRA CHAVES FILHO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : GILDACIO PEREIRA CHAVES FILHO
DATE: 05/03/2021
TIME: 14:30
LOCAL: Online (REMOTA)
TITLE:

A DEFINIR

KEY WORDS:

Regenerative medicine; biocompatibility; health biotechnology.

PAGES: 106
BIG AREA: Ciências Biológicas
AREA: Genética
SUMMARY:

Health biotechnology and regenerative medicine seek alternatives to circumvent chronic or degenerative diseases, including diseases related to bone metabolism and obesity, for which conventional treatments have yet to provide satisfactory solutions. Thus, scientists have been working on the development of treatments based on biomaterials, cells and biomolecules to respond to these problems. Seaweed is a source of new molecules with potential bioactivity. Within this wide variety of molecules, sulfated polysaccharides (SP), which are highly complex and heterogeneous macromolecules, have shown interesting biological activities. Among the different types of seaweed, green seaweeds are the least studied, although several biological activities have already been described for their molecules. With regard to activities in the modeling of cell differentiation, studies with SP of green algae are particularly scarce. Thinking about that, this work evaluated the osteogenic and antiadipogenic activity of SP-containing SP samples obtained from the green marine algae Caulerpa prolifera and Caulerpa sertularioides. Additionally, as an essential part of the evaluation of the biocompatibility of new molecules, the genotoxicity of samples with biological activity was also determined. The results indicate that the SP-rich samples of C. prolifera and C. sertularioides induce differentiation of human stromal stem cells in non-cytotoxic concentrations (<10 µg / mL), promoting an increase in the alkaline phosphatase activity (ALP) and mineralization of the extracellular matrix, without genotoxicity under the conditions tested. The sample containing SP isolated from C. prolifera was also shown to inhibit adipogenic differentiation of pre-adipocytes, in non-cytotoxic concentrations (<200 µg / mL), and to reduce the accumulation of total lipids and triglycerides in the C. elegans model. Taken together, these results highlight the potential of SP in the development of alternative therapies for problems related to bone regeneration and obesity.

BANKING MEMBERS:
Externo ao Programa - 2220417 - CARLOS AUGUSTO GALVAO BARBOZA
Interna - 2492944 - CAROLINE ADDISON CARVALHO XAVIER DE MEDEIROS
Externo ao Programa - 1544647 - MATHEUS DE FREITAS FERNANDES PEDROSA