Banca de DEFESA: SINARA CYBELLE TURIBIO E SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : SINARA CYBELLE TURIBIO E SILVA
DATE: 15/02/2021
TIME: 09:00
LOCAL: ONLINE (REMOTA
TITLE:

ATRAZINE REMOVAL BY Bacillus megaterium CCT 7935 AND Penicillium chrysogenum NRRL 807 FOR BIOREMEDIATION OF CONTAMINATED SOILS

KEY WORDS:

Atrazine; biodegradation; bioremediation; Bacillus megaterium; Penicillium chrysogenum; bioprocess.

PAGES: 116
BIG AREA: Engenharias
AREA: Engenharia Química
SUMMARY:

Atrazine is a herbicide that is still commonly used worldwide, even though it is recognized as a potent chemical-endocrine disruptor capable of causing damage to human health and the environment. Due to its high mobility and persistence, residues are still found in soils and waters. Therefore, the present Thesis aims to analyze the potential of atrazine degradation by Bacillus megaterium CCT 7935 and Penicillium chrysogenum NRRL 807, through bench experiments and in microcosms with soil, for bioremediation of contaminated areas. For data treatment, Single Factor Analysis of Variance (ANOVAOne Way) and Tukey test (p <0.05) were used. B. megaterium CCT 7935 was able to degrade atrazine in bench scale using it as a nitrogen source, especially when submitted to high initial atrazine concentrations (100 and 200 mg/L), with degradation efficiency values greater than 60%. P. chrysogenum NRRL 807 also showed great potential. Both fungusfree and immobilized forms presented degradation efficiency values above 40% when submitted to the initial concentration of 25 mg/L of atrazine, that was used as a carbon source. The removal processes were led by the citrocomo P450 enzyme complex. B. megaterium CCT 7935 and P. chrysogenum NRRL 807 were also efficient in the bioremediation of atrazine in microcosms with soil, with degradation efficiency values above 75%. Both strains used degradation metabolites as sources of nutrientes, however only B. megaterium CCT 7935 appeared to be able to make consecutive and nonsimultaneous use. Although P. chrysogenum has a small advantage in removal efficiency, it inhibited the natural microbiota, that demonstrated too, in turn, good performance in the treatment of atrazine (degradation efficiency of 76%). The results obtained by this Thesis consolidate the potential of the bioprocess developed by the microorganisms of the study in the atrazine treatment, and the bioremediation of soils is configured as an example of the several possible applications, which can also be extended to water and effluent treatment technologies.

BANKING MEMBERS:
Externo à Instituição - CARLOS EDUARDO DE ARAÚJO PADILHA
Externo ao Programa - 1346198 - EVERALDO SILVINO DOS SANTOS
Interno - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Presidente - 347401 - GORETE RIBEIRO DE MACEDO
Externo à Instituição - PEDRO FERREIRA DE SOUZA FILHO