Banca de DEFESA: ANANÍLIA MEDEIROS GOMES DA SILVA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : ANANÍLIA MEDEIROS GOMES DA SILVA
DATE: 23/10/2020
TIME: 10:00
LOCAL: REMOTA (ONLINE)
TITLE:

Regulation of non-coding RNAs in cardiac biological processes and Atrial Fibrillation.

KEY WORDS:

Atrial fibrillation; miRNA; lncRNAs; circRNAs; cardiomyocytes; mRNA; Anti-miR; Mimic-miR.

PAGES: 141
BIG AREA: Ciências da Saúde
AREA: Medicina
SUMMARY:

Advances in molecular, cellular and genetic levels have provided increasingly new discoveries that allow the gradual understanding of key mechanisms of cardiac activity and related pathophysiologies. In this perspective, several cell-signaling pathways are activated for the process of contractility, electrical conduction and vascularization to occur effectively. However, external or endogenous factors can signal significant changes in natural biological processes, leading to cardiac pathophysiology. Among the various diseases that affect the heart, Atrial Fibrillation, AF, is highlighted as one of the main types of arrhythmia most prevalent worldwide. The diagnosis is made by ECG, however the asymptomatology of many patients and the short duration of fibrillation hamper early detection, leading to late diagnoses. Transcriptomic analyzes show that non-coding RNAs play an important modulatory activity, such as miRNAs, which regulate gene expression of mRNA and play a promising role as potential targets for the diagnosis, prognosis and treatments of AF. The activity of miRNAs, in turn, can be regulated by other non-coding RNAs, including lncRNAs and circRNAs, which perform among other functions the activity of capturing miRNAs so that gene expression can be modulated, featuring an activity called competitive endogenous RNA. The activity of miRNAs in AF and of lncRNAs and circRNAs acting on the biological processes involved in this disease were part of the main objectives of this study, in order to obtain a greater understanding of the mechanisms with which they are associated. Scientific articles based on findings about these activities in AF, including the description of the main circulating miRNAs in AF in addition to the miRNAs associated with pathological processes related to this disease, were carried out through database searches. These articles allowed a greater conception about the importance of miRNAs in the pathophysiology of this disease and other associated conditions. Additionally, in an article related to the objective of this study, the expression of circulating miRNAs in the AF event was experimentally evaluated, characterizing some potential targets differently expressed in this condition. Based on these findings, the functional activity of one of the miRNAs differently expressed in this previous study, miR-133b, in the activity of cardiac cells, was demonstrated using in vitro techniques of inhibition, suggesting that this miRNA modulates the expression of some mRNAs expressed in the heart in cardiomyocyte models and that could be regulated by different lncRNAs and circRNAs. Complementarily, through search in database, the main lncRNAs and circRNAs involved in cardiac processes associated with the pathophysiology of AF were analyzed and described, demonstrating that these molecules play a crucial role in the pathological complexity of this disease, whether with a protective or stimulating activity of progression of AF.

BANKING MEMBERS:
Externo à Instituição - ANDRÉ ASSIS LOPES DO CARMO - UFMG
Externa ao Programa - 1055045 - MARCELA ABBOTT GALVAO URURAHY
Externa à Instituição - NATHALIA KELLY DE ARAUJO - IFRN
Externo à Instituição - ROBERT POGUE - UCB
Presidente - 1804884 - VIVIAN NOGUEIRA SILBIGER