Banca de QUALIFICAÇÃO: MELYSSA LIMA DE MEDEIROS

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : MELYSSA LIMA DE MEDEIROS
DATA : 28/06/2019
HORA: 14:30
LOCAL: Sala de Reuniões do Centro de Biociências/UFRN
TÍTULO:

Evaluation of copaiba oil loaded into SNEDDS system applied on wound healing therapeutic processes

PALAVRAS-CHAVES:

Copaiba oil; SNEDDS-type nanoemulsion systems; wound healing therapeutic processes.

PÁGINAS: 100
GRANDE ÁREA: Ciências da Saúde
ÁREA: Medicina
RESUMO:

The management of chronic wound comprises an important aspect of medical practice and in most cases, requires specific treatment for its success. Historical records revealed that copaiba oil had been used primarily to promote wound healing and treat inflammatory and infectious diseases. However, the low solubility in water has limited its pharmacological use. In this study self-nanoemulsion systems SNEDDS-type based on copaiba oil (CO) were prepared in the presence of Tween® 80 at aqueous medium (neutral and saline), aiming the CO-lower (0.5%) content loaded into SNEDDS systems (self-nanoemulsifying drug delivery system) for CO-gradual and prolonged release applied on wound healing or diabetic wound healing. Physical-chemistry analysis was applied for characterize both in natura CO and SNEDDS-systems. Chemical approach afforded new formulations prepared without co-surfactant [79% of water, 20% of surfactant, 1% of an oil phase mixture containing (1:1) CO and sunflower oil], which justify its foremost importance on CO-pharmacologic applications. The animals were subjected to dorsal cutaneous skin flap surgery and received post-operative topically treatment daily with the CO-SNEDDS-systems. The wound healing in vivo assay was developed for Wistar (Rattus novergicus albinus) divided in groups of five animals each (n=5). Histopathological and immunohistochemical analysis were performed after 7 to 14 treatment days for animal´s groups. Histomorphometric analysis revealed a smaller number of neutrophils and lymphocytes in the treated group (7 days) as well as collagenesis, fibroblast proliferation and lager epithelial thickness that were more evident in the treated group (14 days). Keratinocytes with increased immunoreactivity for IL-1β in the control group contributed to the repair delay. The greater number of FGF-2 immunostained cells were associated with positive collagenesis and accelerated the wound healing process. In addition, a comparative in vivo approach concerning to in experimental model of cutaneous lesions were assessed, of which the effect of low-level laser therapy on angiogenesis and matrix metalloproteinase-2 immunoexpression in wound repair was investigated in the absence or/and presence of the CO-SNEDDS-system.

MEMBROS DA BANCA:
Interno - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externo ao Programa - 2941160 - JOSE HERIBERTO OLIVEIRA DO NASCIMENTO
Presidente - 1199127 - SILVIA REGINA BATISTUZZO DE MEDEIROS