Banca de QUALIFICAÇÃO: FRANCISCO JOSÉ CARVALHO MOREIRA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : FRANCISCO JOSÉ CARVALHO MOREIRA
DATA : 08/06/2018
HORA: 09:00
LOCAL: Sala SS1 do Depto. de Biologia Ceular e Genética / Centro de Biociências
TÍTULO:

Prospecting and Development of an Autonanoemulsifying System (SNEDDS) with extracts of Tephrosia toxicaria Pers. for the Alternative Control of Root-Knot Nematodes.

PALAVRAS-CHAVES:

Timbó, Meloidogyne, bioactive molecules, nanoencapsulation, ecotoxicity, cytotoxicity.

PÁGINAS: 100
GRANDE ÁREA: Ciências Biológicas
ÁREA: Biologia Geral
RESUMO:

Among the phytonematoids, the genus Meloidogyne Goeldi (1887) stands out due to its genetic plasticity, versatility and adaptability, which places them as the main group causing damage to agriculture. Several compounds of plant origin have nematicidal activity, are profitable and environmentally safe, and can be used in organic farming systems, however, many of these products degrade easily, reducing persistence, making control difficult. In this sense, the nanoencapsulation of botanical pesticides presents as a formidable technology, being able to transform the physical state of the substances for better manipulation, allowing the optimization of formulations with controlled release, thus avoiding their degradation, due to adverse conditions. Thus, the objective of this work was the prospection and development of an autonanoemulsifying system (SNEDDS) with extracts of Tephrosia toxicaria for the control of the root-knot nematodes. In order to do so, five experiments were established in which the nematicidal activity in vitro and in vivo work on Tephrosia toxicaria extracts were evaluated. Next, a multi-species environmental safety test group was carried out, being aquatic ecotoxicity in microcrustaceans (Ceriodaphinia dubia and Moina micrura); cytotoxicity in 3T3 cells; soil ecotoxicity in Caenorhabditis elegans and phytotoxicity in Lactuca sativa. The aqueous and hydroethanolic extracts of the roots of T. toxicaria that had the best effect for root-knot nematodes were characterized physicochemical. Subsequently the extract of the roots and their derivatives were incorporated into an autonemulsifiable system (SNEDDS), which were characterized and then carried out tests of nematicidal activity in vitro and environmental safety in microcrystalline (C. dubia) and phytotoxicity in Lactuca sativa . The results demonstrate that the aqueous extracts of T. toxicaria showed nematicidal effect in the in vitro and in vivo assays; it was also observed that the extracts had no deleterious effect on the 3T3 cells and on the germination of L. sativa; there was a small reduction in survival and reproduction, but no significant change in the behavior of C. elegans was observed, while for microcrustaceans, the same were affected by root extract, causing 100% mortality. As for the SNEDDS of the root extracts, they maintained the nematicidal effect in vitro, and, not affecting the germination of L. sativa nor the survival of C. dubia. The data set indicate the extracts of T. toxicaria as an alternative to the control of root-knot nematodes, with significant effect at low concentrations and with reasonable environmental safety. The SNEDDS system demonstrated potential as a new input for the management of root-knot nematodes, which resulted in a patent filing with the National Institute of Industrial Property (INPI), thus a formulation that could be used commercially in agriculture.

MEMBROS DA BANCA:
Presidente - 1251018 - RIVA DE PAULA OLIVEIRA
Interno - 373.201.654-49 - MARIA APARECIDA MEDEIROS MACIEL - UFRN
Externo ao Programa - 1228866 - EDUARDO LUIZ VOIGT