Banca de QUALIFICAÇÃO: FRANCISCO BRUNO FERREIRA DE FREITAS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : FRANCISCO BRUNO FERREIRA DE FREITAS
DATE: 20/10/2023
TIME: 14:00
LOCAL: Via Google Meet
TITLE:

BIODEGRADATION OF 2-METHYL-NAPHTHALENE AND PHEANTHRENE BY PSEUDOMONAS AERUGINOSA IN MICROCOSMS

KEY WORDS:

Biodegradation, phenanthrene, 2-methyl-naphthalene, Pseudomonas aeruginosa, biosurfactants.

PAGES: 48
BIG AREA: Engenharias
AREA: Engenharia Química
SUBÁREA: Processos Industriais de Engenharia Química
SPECIALTY: Processos Bioquímicos
SUMMARY:

Oil stands out as one of the main raw materials for modern society, being able to contribute to the constitution of materials ranging from fuels to medicines. Knowing this great importance, there is an increasing search for its extraction and refinement, which ends up causing spills in soils and oceans. Because of their carcinogenicity and mutagenic aspects, some polycyclic aromatic hydrocarbons (PAHs) pose risks to humans and wildlife, such as 2-Methylnaphthalene (2-MN) and phenanthrene. Therefore, knowing the problems that petroleum and its hydrocarbons can cause, it is necessary to seek solutions, one of the most studied of which is the biodegradation of petroleum compounds in marine environments, which is based on the ability of microorganisms to degrade petroleum compounds. Microorganisms capable of degrading such compounds may include bacteria such as Enterobacter sp., Pseudomonas and Rhodococcus and fungi and algae. On the other hand, a factor that hinders the microbiological decomposition of oils and derivatives, when an oil spill occurs at sea, is the hydrophobicity of hydrocarbon molecules, which is why the industry invests in the development of dispersants that reduce surface tension at the hydrocarbon interface. /water, facilitating their movement in the waves and with the capacity to generate more small droplets. In this context, the literature has demonstrated the advantages of biosurfactants (surfactants of biological origin) in relation to surfactants from the chemical industry. Thus, the main objective of the present study is to evaluate the biodegradation capacity of 2-Methyl-Naphthalene and Phenanthrene (model hydrocarbons) by Pseudomonas aeruginosa in the presence of industrial biosurfactant (dispersant) with cultivation in microcosms. The biosurfactant (dispersant) will be provided by a chemical industry, and will be characterized based on surface tension and emulsification index analyses. Subsequently, the degradation of the hydrocarbons will be carried out, varying the concentration of the hydrocarbons and the biosurfactant, for 10 days, monitoring pH, bacterial growth, concentration of rhamnolipids, concentration of total proteins, in addition to the activity of laccase, manganese peroxidase and peroxidase enzymes. It is noteworthy that the degradation of the mixture of 2-Methylnaphthalene (2-MN) and phenanthrene will also be evaluated, with the aim of evaluating the strain's ability to degrade mixtures of hydrocarbons.

COMMITTEE MEMBERS:
Interno - 3304882 - CARLOS EDUARDO DE ARAÚJO PADILHA
Presidente - 1346198 - EVERALDO SILVINO DOS SANTOS
Interna - 3214434 - NATHALIA SARAIVA RIOS