Banca de QUALIFICAÇÃO: WILZA KIMILLY VITAL DE PAIVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : WILZA KIMILLY VITAL DE PAIVA
DATE: 30/12/2022
TIME: 14:00
LOCAL: Via Google Meet
TITLE:

SURFACTIN PRODUCTION BY Bacillus subtilis UFPEDA 438 FROM SUGARCANE MOLASSES AND EVALUATION OF ITS BIOLOGICAL ACTIVITIES INCLUDING IN SILICO AGAINST SARS-CoV-2

KEY WORDS:

B. subtilis; Bactericide; Biosurfactant; Fungicide; Surfactin

PAGES: 81
BIG AREA: Engenharias
AREA: Engenharia Química
SUBÁREA: Processos Industriais de Engenharia Química
SPECIALTY: Processos Bioquímicos
SUMMARY:

Lipopeptides are biosurfactants, substances with surfactant character where each family corresponds to a group of isoforms that differ in the composition of the peptide, the length of the lipid chain, and the bond between them. Among them, stand out Fengicine, Iturin and Surfactin, and lipopeptides with high surfactant and antibiotic potential. As it is a lipopeptide, surfactin is a set of isoforms, biosynthesized or engineered, in which the relationship between their structures and properties helps in choosing and directing the product to the final application, ranging from the surface cleaning and disinfection industry crude oil recovery, in addition to being the subject of studies in medical and pharmacological applications. In the context of COVID-19, the present study aims to produce surfactin and iturin through the cultivation of Bacillus subtilis UFPEDA 438, using sugarcane molasses as a substrate, as well as to evaluate its biological activity including its antiviral potential through in silico analysis of the extract produced against the 4 main target proteins of SARS-CoV-2. The results showed that 8 isoforms of surfactin and 2 of iturin were present in the samples, obtaining a maximum productivity of 148.548 ± 4.672 mg/L of surfactin. The extracts produced in both steps showed satisfactory levels and emulsification activities and promoted a reduction in the surface tension of water by up to 55.24%. Against free radicals (ROS), the samples also managed to block about 41.95% of them. Regarding the in silico evaluation, the greatest interaction of the studied biosurfactants was with the Spyke protein, which is crucial for the binding of the virus with the host human cell.

COMMITTEE MEMBERS:
Presidente - 1346198 - EVERALDO SILVINO DOS SANTOS
Interna - 3214434 - NATHALIA SARAIVA RIOS
Externa ao Programa - 2378605 - CRISTIANE FERNANDES DE ASSIS - UFRNExterno ao Programa - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR - UFRN