Banca de DEFESA: JÚLIA CAROLINE RIBEIRO DE CARVALHO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JÚLIA CAROLINE RIBEIRO DE CARVALHO
DATE: 15/07/2022
TIME: 15:00
LOCAL: Virtual
TITLE:

Recovery and purification of invertases from Saccharomyces cerevisiae in microchannels using PEG 1500/magnesium sulfate aqueous two-phase system

KEY WORDS:

Invertase, aqueous two-phase system, liquid-liquid extraction, microchannel, flow pattern, polyethylene glycol.

PAGES: 85
BIG AREA: Engenharias
AREA: Engenharia Química
SUBÁREA: Processos Industriais de Engenharia Química
SPECIALTY: Processos Bioquímicos
SUMMARY:

The search for process intensifying methods is one of the targets of chemical engineering, including in classical processes such as liquid-liquid extraction. The separation of biomolecules uses this type of extraction and the search for intensification has gained prominence in the literature. However, studies on the evaluation of the impact of flow patterns on extraction performance are still scarce, especially in complex environments with multiple target molecules such as the products of cell lysis. Thus, in this work, invertase from Saccharomyces cerevisiae was separated using the liquid-liquid extraction technique in a microsystem through a aqueous two-phase system (ATPS) formed by polyethylene glycol (PEG) 1500 (15% w/w), magnesium sulfate ( 20% w/w) and distilled water (65% w/w). The study was carried out using different operating conditions, including variations in total flow from 9.30 μL/min to 324.45 μL/min, volumetric ratio between top and bottom phases from 1:2 to 2:1, diameter of 0.51 to 2.06 mm and tube lengths from 0.58 to 9.40 m. The arrangement of the phases was observed during the course in the microsystem using a wide range of total flow and a flow pattern map was obtained. The patterns observed were short-slug, slug and parallel, with the short-slug pattern for low flows and parallel for high flows. It was found that increasing the total flow negatively affected the recovery of invertase during extraction, while higher values of residence time and volumetric ratio improved the recovery and the enzyme purification factor. Using 35 min residence time, 2:1 volumetric ratio and 1.14 mm tube diameter, the short-slug and slug patterns showed the best process performances. These results reached, numerically, the values of 53.95% and 51.15% of invertase recovery and purification factor of 1.84 and 2.78, respectively. The effect of the channel diameter was also studied and it was concluded that in smaller diameters, the recovery obtained better results, highlighting the value of 57.01% of invertase recovery and a purification factor of 4.16 in the 0. 51 mm diameter when the residence time was 35 min and the volumetric ratio was 2:1. The best result can also be justified by the value of the maximum volumetric mass transfer coefficient (kLa) obtained of 8.86 x 10^-4s^-1 when compared with the resulting values for the other operating conditions. Thus, the present study contributes to the development of knowledge in the area of microsystems in problems of enzyme separation through liquid-liquid extraction.

COMMITTEE MEMBERS:
Presidente - 1584174 - DOMINGOS FABIANO DE SANTANA SOUZA
Interno - 1346198 - EVERALDO SILVINO DOS SANTOS
Interna - 3214434 - NATHALIA SARAIVA RIOS
Externo à Instituição - CARLOS EDUARDO DE ARAÚJO PADILHA - UFRN