Banca de DEFESA: LUAN TALES COSTA DE PAIVA VASCONCELOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : LUAN TALES COSTA DE PAIVA VASCONCELOS
DATA : 23/02/2018
HORA: 14:00
LOCAL: SALA DO CATRE
TÍTULO:

"Induction strategies of the recombinant 503 antigen of Leishmania i. chagasi expressed in M15 Escherichia coli"

PALAVRAS-CHAVES:

503 antigen, recombinant Escherichia coli, Leishmaniosis, induction.

PÁGINAS: 75
GRANDE ÁREA: Engenharias
ÁREA: Engenharia Química
RESUMO:

With nearly 900,000 to 1.3 million new cases annually, Leishmaniosis is a complex of diseases that can be fatal if not given proper attention. Despite its relevance in the public health system, there is no vaccine capable of preventing disease in humans so far, and its treatment is expensive and aggressive to human health. Vaccination remains the most practical and realistic way of fight against the disease, thus, the production of recombinant antigens as a pathway towards a vaccine acquisition is necessary. In addition, the optimization of the strategy of the recombinant antigen’s production is of interest so it can make the process viable. Therefore, the present study aims to optimize the induction parameters of the 503 Leishmania i. chagasi antigen expressed in recombinant Escherichia coli. Hence, the induction at different cell densities (optical density at 600 nm of 0.5 and 1.0) was evaluated in order to analyse the influence of the induction time on the yield of the protein of interest. In this segment, lactose and isopropyl-β-D-thiogalactopyranoside (IPTG) were used as inducer molecules, using various concentrations: 0.1 g/L, 1.0 g/L and 10 g/L for lactose and 20 μM, 100 μM, 500 μM and 1000 μM for IPTG. The results presented that the concentration of IPTG that obtained the higher antigen levels was that of 100 μM (0.087 g/L), a 10-fold lower concentration than was being previously used in this type of system, and for lactose it was 1 g/L (0.016 g/L). Thus, the induction with 100 μM of IPTG allowed to obtain the antigen with a concentration 5.6 times higher than the lactose induction maximum concentration.

 

 

 

MEMBROS DA BANCA:
Presidente - 1346198 - EVERALDO SILVINO DOS SANTOS
Externo ao Programa - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Externo à Instituição - ANDREA FARIAS DE ALMEIDA - UFPB
Externo à Instituição - NATHALIA KELLY DE ARAUJO - UFRN