Universidade Federal do Rio Grande do Norte Natal, 18 de Maio de 2024

Resumo do Componente Curricular

Dados Gerais do Componente Curricular
Tipo do Componente Curricular: MÓDULO
Unidade Responsável: PROGRAMA DE PÓS-GRADUAÇÃO EM NEUROCIÊNCIAS (10.89.00.05)
Código: NEU0049
Nome: BIOLOGIA MOLECULAR NAS NEUROCIÊNCIAS
Carga Horária Teórica: 60 h.
Carga Horária Prática: 0 h.
Carga Horária de Ead: 0 h.
Carga Horária Total: 60 h.
Pré-Requisitos:
Co-Requisitos:
Equivalências:
Excluir da Avaliação Institucional: Não
Matriculável On-Line: Sim
Horário Flexível da Turma: Não
Horário Flexível do Docente: Sim
Obrigatoriedade de Nota Final: Sim
Pode Criar Turma Sem Solicitação: Não
Necessita de Orientador: Não
Exige Horário: Sim
Permite CH Compartilhada: Não
Quantidade de Avaliações: 1
Ementa/Descrição: Engenharia genética nas neurociências: Clonagem e produção de plasmídeos; Vetores retrovirais, lentivirais e adeno-associados; Transfecção celular in vitro e in vivo; Hibridização in situ; "Knockouts" integrais e condicionais (CreLox); "Knockin" e transgênicos; TALEN; CRISPR; RNA de interferência; "Microarrays"; Sequenciamento em larga escala; ChIP.
Referências: 1) Genes IX - Artmed, Ed.9, 2009. Autor: Edward Lewin 2) From Molecules to Networks, Second Edition: An Introduction to Cellular and Molecular Neuroscience - Acdemic Press. Editor: Ruth Heidelberger 3) Artigos científicos (Gorski et al., 2002;Noctor et al., 2004;Arlotta et al., 2005;Takahashi and Yamanaka, 2006;Berninger et al., 2007;Takahashi et al., 2007;Wickersham et al., 2007;Alcamo et al., 2008;Aoi et al., 2008;Arlotta et al., 2008;Kubrusly et al., 2008;Wernig et al., 2008;Bergami et al., 2009;Heinrich et al., 2010;McKenna et al., 2011;Franco et al., 2012;Karow et al., 2012;Verderio et al., 2012;De la Rossa et al., 2013;Deshpande et al., 2013;Zhang et al., 2013;Gao, 2014;Vasistha et al., 2014) Alcamo, E.A., Chirivella, L., Dautzenberg, M., Dobreva, G., Farinas, I., Grosschedl, R., and Mcconnell, S.K. (2008). Satb2 regulates callosal projection neuron identity in the developing cerebral cortex. Neuron 57, 364-377. Aoi, T., Yae, K., Nakagawa, M., Ichisaka, T., Okita, K., Takahashi, K., Chiba, T., and Yamanaka, S. (2008). Generation of pluripotent stem cells from adult mouse liver and stomach cells. Science 321, 699-702. Arlotta, P., Molyneaux, B.J., Chen, J., Inoue, J., Kominami, R., and Macklis, J.D. (2005). Neuronal subtype-specific genes that control corticospinal motor neuron development in vivo. Neuron 45, 207-221. Arlotta, P., Molyneaux, B.J., Jabaudon, D., Yoshida, Y., and Macklis, J.D. (2008). Ctip2 controls the differentiation of medium spiny neurons and the establishment of the cellular architecture of the striatum. J Neurosci 28, 622-632. Bergami, M., Berninger, B., and Canossa, M. (2009). Conditional deletion of TrkB alters adult hippocampal neurogenesis and anxiety-related behavior. Commun Integr Biol 2, 14-16. Berninger, B., Costa, M.R., Koch, U., Schroeder, T., Sutor, B., Grothe, B., and Gotz, M. (2007). Functional properties of neurons derived from in vitro reprogrammed postnatal astroglia. J Neurosci 27, 8654-8664. De La Rossa, A., Bellone, C., Golding, B., Vitali, I., Moss, J., Toni, N., Luscher, C., and Jabaudon, D. (2013). In vivo reprogramming of circuit connectivity in postmitotic neocortical neurons. Nat Neurosci 16, 193-200. Deshpande, A., Bergami, M., Ghanem, A., Conzelmann, K.K., Lepier, A., Gotz, M., and Berninger, B. (2013). Retrograde monosynaptic tracing reveals the temporal evolution of inputs onto new neurons in the adult dentate gyrus and olfactory bulb. Proc Natl Acad Sci U S A 110, E1152-1161. Franco, S.J., Gil-Sanz, C., Martinez-Garay, I., Espinosa, A., Harkins-Perry, S.R., Ramos, C., and Muller, U. (2012). Fate-restricted neural progenitors in the mammalian cerebral cortex. Science 337, 746-749. Gao, P., Postiglione, M.P., Krieger, T.G., Hernandez, L., Wang, C., Han, Z., Streicher, C., Papusheva, E., Insolera, R., Chugh, K., Kodish, O., Huang, K., Simonns, B.D., Luo, L., Hippenmeyer, S., and Shi, S. (2014). Deterministic Progenitor Behavior and Unitary Production of Neurons in the Neocortex. Cell 159. Gorski, J.A., Talley, T., Qiu, M., Puelles, L., Rubenstein, J.L., and Jones, K.R. (2002). Cortical excitatory neurons and glia, but not GABAergic neurons, are produced in the Emx1-expressing lineage. J Neurosci 22, 6309-6314. Heinrich, C., Blum, R., Gascon, S., Masserdotti, G., Tripathi, P., Sanchez, R., Tiedt, S., Schroeder, T., Gotz, M., and Berninger, B. (2010). Directing astroglia from the cerebral cortex into subtype specific functional neurons. PLoS Biol 8, e1000373. Karow, M., Sanchez, R., Schichor, C., Masserdotti, G., Ortega, F., Heinrich, C., Gascon, S., Khan, M.A., Lie, D.C., Dellavalle, A., Cossu, G., Goldbrunner, R., Gotz, M., and Berninger, B. (2012). Reprogramming of pericyte-derived cells of the adult human brain into induced neuronal cells. Cell Stem Cell 11, 471-476. Kubrusly, R.C., Panizzutti, R., Gardino, P.F., Stutz, B., Reis, R.A., Ventura, A.L., De Mello, M.C., and De Mello, F.G. (2008). Expression of functional dopaminergic phenotype in purified cultured Muller cells from vertebrate retina. Neurochem Int 53, 63-70. Mckenna, W.L., Betancourt, J., Larkin, K.A., Abrams, B., Guo, C., Rubenstein, J.L., and Chen, B. (2011). Tbr1 and Fezf2 regulate alternate corticofugal neuronal identities during neocortical development. J Neurosci 31, 549-564. Noctor, S.C., Martinez-Cerdeno, V., Ivic, L., and Kriegstein, A.R. (2004). Cortical neurons arise in symmetric and asymmetric division zones and migrate through specific phases. Nat Neurosci 7, 136-144. Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S. (2007). Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861-872. Takahashi, K., and Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676. Vasistha, N.A., Garcia-Moreno, F., Arora, S., Cheung, A.F., Arnold, S.J., Robertson, E.J., and Molnar, Z. (2014). Cortical and Clonal Contribution of Tbr2 Expressing Progenitors in the Developing Mouse Brain. Cereb Cortex. Verderio, C., Cagnoli, C., Bergami, M., Francolini, M., Schenk, U., Colombo, A., Riganti, L., Frassoni, C., Zuccaro, E., Danglot, L., Wilhelm, C., Galli, T., Canossa, M., and Matteoli, M. (2012). TI-VAMP/VAMP7 is the SNARE of secretory lysosomes contributing to ATP secretion from astrocytes. Biol Cell 104, 213-228. Wernig, M., Zhao, J.P., Pruszak, J., Hedlund, E., Fu, D., Soldner, F., Broccoli, V., Constantine-Paton, M., Isacson, O., and Jaenisch, R. (2008). Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson's disease. Proc Natl Acad Sci U S A 105, 5856-5861. Wickersham, I.R., Finke, S., Conzelmann, K.K., and Callaway, E.M. (2007). Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat Methods 4, 47-49. Zhang, Y., Pak, C., Han, Y., Ahlenius, H., Zhang, Z., Chanda, S., Marro, S., Patzke, C., Acuna, C., Covy, J., Xu, W., Yang, N., Danko, T., Chen, L., Wernig, M., and Sudhof, T.C. (2013). Rapid single-step induction of functional neurons from human pluripotent stem cells. Neuron 78, 785-798.

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