Banca de QUALIFICAÇÃO: RAISSA DAS GRAÇAS PONTES ARAÚJO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : RAISSA DAS GRAÇAS PONTES ARAÚJO
DATE: 01/02/2024
TIME: 14:00
LOCAL: Via Google Meet
TITLE:

USE OF SHRIMP POND WATER FOR CULTIVATION OF MICROALGAE Synechococcus nidulans AIMING FOR BIOMASS PRODUCTION

KEY WORDS:

Shrimp farming; sugar cane molasses; Biorefinery; Synechococcus nidulans; Phycocyanin.

PAGES: 99
BIG AREA: Engenharias
AREA: Engenharia Química
SUBÁREA: Processos Industriais de Engenharia Química
SPECIALTY: Processos Bioquímicos
SUMMARY:

The shrimp farming activity refers to the farming of shrimp and is very significant in the northeastern region of Brazil. To ensure the functioning of farms, an abundance of water is needed, which, if improperly disposed of, can cause various environmental damages. Another prominent activity in the country is the cultivation of sugar cane, which generates several by-products. One of them is molasses, whose sugar and nutrient levels are quite high. Thus, the use of microalgae to aid in the treatment of wastewater linked to the biorefinery process has been gaining prominence. Studies show that sugarcane molasses can be used as an economical and efficient supplement to microalgae growing media, offering substantial amounts of organic carbon. This work sought to evaluate the potential for reusing water from the organic shrimp production pond to dilute the Zarrouk medium (50%, 60%, and 70%) and the effect of adding molasses, compared with the traditional Zarrouk medium for cyanobacteria cultivation. Synechococcus nidulans, as low-cost cultivation media can enable the production of microalgae on large scales in an optimized way. It also sought to extract phycocyanin, a natural pigment present in microalgae of the genus Synechococcus. Cultivations were carried out in duplicate, under autotrophic and mixotrophic conditions, with a 12-hour light/dark photoperiod at 25°C and with mechanical air aeration. Cultivations of Zarrouk 70% SM (Without Molasses) and CM (With Molasses) generally showed better results compared to other diluted Zarrouk media, with biomass concentrations of 1.1005 g/L ± 0.1789 g/L and 0.9860 g /L± 0.0206 g/ L, respectively, but with lower results compared to traditional Zarrouk. It was found that the addition of carbon to the medium did not significantly interfere in favoring greater production. The pH of the medium remained in the range of 8.27-9.92. Biomass was characterized by carbohydrates, lipids, crude protein, and carotenoids. Carbohydrates were favored by the addition of molasses and the 70% CM test reached a maximum percentage of 21.24% ± 4.44%, while the lipid content was not affected so that it presented maximum values of 36.60% ± 0.00% (70% SM) and 31.80% ± 2.26% (70% CM). The protein content was also not affected, with maximum values reaching 21.55% ± 0.43% (70% SM) and 19.37% ± 0.56% (70% SM). Regarding the carotenoid content, a slight increase in concentration was observed in the CM experiments, with the highest value of 135.2238 mg/g ± 25.9163 mg/g (70% CM). The same occurred with phycocyanin, which was possible to extract and presented a concentration of 1.4919 mg/mL ± 0.2898 mg/mL in the 70% CM assay, with Extract Purity (PE) of 0.29.

COMMITTEE MEMBERS:
Presidente - 1346198 - EVERALDO SILVINO DOS SANTOS
Interno - 3304882 - CARLOS EDUARDO DE ARAÚJO PADILHA
Externa ao Programa - ***.713.784-** - BRUNA MARIA EMERENCIANO DAS CHAGAS - UFRN
Externo ao Programa - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR - UFRN