Banca de QUALIFICAÇÃO: STEPHANIE CAROLINE BIVAR MATIAS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : STEPHANIE CAROLINE BIVAR MATIAS
DATE: 19/07/2021
TIME: 14:00
LOCAL: Via Google Meet
TITLE:

EVALUATION OF PRODUCTION STRATEGIES IN DISCONTINUOUS CULTIVATION OF Toxoplasma Gondii MULTI-ANTIGENIC CHIMERIC PROTEIN TgAGSS/BsT IN Escherichia coli

KEY WORDS:

Toxoplasmosis, Recombinant Escherichia coli, discontinuous culture, chimeric protein

PAGES: 85
BIG AREA: Engenharias
AREA: Engenharia Química
SUBÁREA: Processos Industriais de Engenharia Química
SPECIALTY: Processos Bioquímicos
SUMMARY:

Despite the advances in science and technology, toxoplasmosis does not yet have a vaccine capable of immunizing humans and animals against all isolated types of T. gondii. The treatment of this disease is based on the use of drugs that have some toxicity and can cause serious side effects justifying the need for the development of vaccines or immunotherapeutic as preventive measures. Due to the great diversity of antigens presented by Toxoplasma gondii, a vaccine and diagnostic kit containing multiple antigens is quite promising. Among the bacteria used to produce recombinant proteins, Escherichia coli is the most widely used because of its well described genetic characteristics. In this context, the present work has as main focus to evaluate discontinuous cultivation strategies in the production of Toxoplasma gondii multi-antigenic chimeric protein TgAGS / BsT performed in a rotary incubator. To achieve the proposed objective, several assays will be performed to observe the kinetic behavior of the E. coli XL-1 Blue strain in five different medium compositions (2xTY, glucose supplemented TB, glycerol supplemented TB, LB and M9) without addition of inductor in rotary incubator. In addition, the stability of the plasmid, the influence of the medium on the kinetic parameters of E. coli cell growth will be evaluated. and TgAGS / BsT multi-antigen chimera protein expression and acetic acid formation.

BANKING MEMBERS:
Presidente - 1346198 - EVERALDO SILVINO DOS SANTOS
Externo à Instituição - CARLOS EDUARDO DE ARAÚJO PADILHA - UFRN
Externa à Instituição - NATHALIA KELLY DE ARAUJO - UFRN