Banca de DEFESA: EDUARDO WAGNER VASCONCELOS DE ANDRADE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : EDUARDO WAGNER VASCONCELOS DE ANDRADE
DATE: 06/08/2020
TIME: 15:00
LOCAL: VIRTUAL
TITLE:

Osmoporation of fisetin into probiotic bacteria cells (Lactobacillus acidophilus): process parameters, bioaccessibility and incorporation into dairy beverage.

KEY WORDS:

dairy fermented products; microencapsulation; biotechnology; functional foods.

PAGES: 110
BIG AREA: Engenharias
AREA: Engenharia Química
SUMMARY:

This study investigates the encapsulation of hydrophobic fisetin into probiotic Lactobacillus acidophilus bacteria cells. Initially, the influence of selected process parameters (fisetin concentration and osmotic pressure) on the encapsulation efficiency was evaluated. Furthermore, the structural characterization (determined by FT-IR and DSC techniques), bioaccessibility of the biocapsules, as well as the acidifying activity performance of L. acidophilus in a dairy beverage (model food) were investigated. Results show that the highest encapsulation efficiency (28%) was achieved at fisetin concentration of 2 mg.ml^-1 and osmotic pressure 15 MPa. Additionally, differential exploratory calorimetry curves (DSC) demonstrated an effective fisetin internalization into cell cytoplasm and its adsorption onto cell surface. The in vitro gastrointestinal simulation showed that the bioaccessibility of encapsulated fisetin was 45.9%, while free fisetin reached only 0.7%. When fisetin biocapsules were added to milk, the fisetin content released during milk fermentation substantially increased the antioxidant activity of the final fermented beverage (95.3%) for 14 days and the encapsulated fisetin content remained high (85%) for 28 days. Overall, the results prove that fisetin osmoporation into L. acidophilus cells is a simple and efficient technique that enables phytoactive protection for food industry applications

BANKING MEMBERS:
Externa à Instituição - ANDREA FARIAS DE ALMEIDA - UFPB
Externo à Instituição - ANTONIO DE ANCHIETA CAMARA JUNIOR
Externa à Instituição - DANIELLA REGINA ARANTES MARTINS SALHA
Externa à Instituição - LOURENA MAFRA VERISSIMO
Externa ao Programa - 1517220 - MARCIA REGINA DA SILVA PEDRINI
Presidente - 1246737 - ROBERTA TARGINO HOSKIN