Banca de QUALIFICAÇÃO: MARCELO DA SILVA PEDRO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : MARCELO DA SILVA PEDRO
DATE: 20/12/2019
TIME: 09:00
LOCAL: na Sala do CATRE
TITLE:

Effect of flow patterns on protein partitioning in aqueous two-phase systems employing microdevices.

KEY WORDS:

BSA, liquid-liquid extraction, flow pattern, microdevice.

PAGES: 58
BIG AREA: Engenharias
AREA: Engenharia Química
SUMMARY:

The present study investigated the effect of flow pattern on biomolecule extraction using aqueous two-phase systems (ATPS) of polyethylene glycol (PEG)/phosphate in a microdevice. Flow patterns were determined for ATPS composed of PEG/dibasic potassium phosphate using different polymer molecular masses (PEG 1500, PEG 4000 and PEG 6000) and silicon minichannels with circular geometry arranged in sine form and 1.8 mm diameter. As expected, the gradual increase in liquid flow allowed to achieve the flow patterns in the following sequence: slug, droplet and parallel flow. The high viscosity of the polymeric phases prevented the occurrence of parallel flow in systems with higher molecular masses of PEG. The study of the effect of the flow pattern on mass transfer was performed for the three flow patterns obtained in the PEG-1500/dibasic potassium phosphate system at residence times of 3, 5 and 10 min for the 1.8 mm minichannel. In addition, microchannel assays with 1.14 mm and 0.510 mm were used employing the droplet flow pattern. The total flow rate ranged from 20 to 700 μL/min. Using a 1.8 mm microchannel and 10 min incubation employing slug, droplet and parallel flow patterns, the recovery efficiencies of bovine serum albumin (BSA) were of 38%, 56% and 32%, respectively. The recovery of a smaller molecule (ampicillin) was tested for comparison with the protein (large molecule) and its partitioning was independent of flow regime. The reduction in channel diameter had a positive effect on extraction efficiency and the highest efficiency value obtained was 69% for the 0.510 mm microchannel with 10 min of residence time. Volumetric mass transfer coefficients were obtained in the range 0.41-2.75x10^-3 (s^-1) for all microchannels and flow patterns.

BANKING MEMBERS:
Externo à Instituição - CARLOS EDUARDO DE ARAÚJO PADILHA - UFRN
Presidente - 1584174 - DOMINGOS FABIANO DE SANTANA SOUZA
Interno - 1346198 - EVERALDO SILVINO DOS SANTOS
Externa ao Programa - 1218001 - LIANA FRANCO PADILHA