Banca de QUALIFICAÇÃO: LUAN TALES COSTA DE PAIVA VASCONCELOS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : LUAN TALES COSTA DE PAIVA VASCONCELOS
DATA : 07/02/2018
HORA: 14:00
LOCAL: SALA DO CATRE
TÍTULO:

"Induction strategies of the recombinant 503 antigen of Leishmania i. chagasi expressed in M15 Escherichia coli"

PALAVRAS-CHAVES:

503 antigen, recombinant Escherichia coli, Leishmaniosis, induction.

PÁGINAS: 75
GRANDE ÁREA: Engenharias
ÁREA: Engenharia Química
RESUMO:

With nearly 900,000 to 1.3 million new cases annually, Leishmaniosis is a complex of diseases that can be fatal if not given proper attention. Despite its relevance in the public health system, there is no vaccine capable of preventing disease in humans so far, and its treatment is expensive and aggressive to human health. The present study aims to optimize the induction parameters of the 503 Leishmania i. chagasi antigen expressed in recombinant Escherichia coli. Hence, lactose (0.1, 1.0 e 10.0 g/L) and isopropyl-β-D-thiogalactopyranoside (IPTG) (20.0; 100.0; 500.0 e 1000.0 mM) were used and analysed as inducer molecules at different cell densities (OD₆₀₀ to 0.5 and 1.0) in order to assess the influence of the induction time on the yield of the protein of interest. The results presented that the IPTG concentration of 100 μM favoured the higher antigen levels (0.087 g/L), being a lower concentration than is usually used in this type of system reported in the literature, and for lactose was 1 g/L (0.016 g/L). Thus, induction with 100.0 mM of IPTG allowed achieving the 503 antigen with a concentration 5.6 times higher than the maximum obtained when lactose was used as an inductor.

 

 

MEMBROS DA BANCA:
Presidente - 1346198 - EVERALDO SILVINO DOS SANTOS
Externo ao Programa - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Externo ao Programa - 1610982 - PRISCILLA MOURA ROLIM MADEIRA